Western blot analysis of alpha Dystroglycan using a alpha Dystroglycan polyclonal antibody (Product #PA5-114320). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:1000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SkBr3 cell lysate, Lane 2: Mouse placenta tissue lysate, Lane 3: Siha cell lysate